NUMB antibody

Catalog No. ABIN324854

The Sheep Polyclonal anti-NUMB antibody has been validated for WB, ELISA and ICC. It is suitable to detect NUMB in samples from Human.

Quick Overview for NUMB antibody (ABIN324854)

Target: NUMB (Numb Homolog (NUMB))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This NUMB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-NUMB Antibody

Specificity: Recognizes human Numb. Species sequence homology: mouse: 88 % and canine: 90 %.

Purification: Immunoaffinity purified

Immunogen: E. coli-derived recombinant human rhNumb.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (1 μg/mL)

Usage: Suitable for use in Western blot, Immunocytochemistry, and Direct ELISA. Western blot: 1.0 μg/mL. Immunocytochemistry: 10 μg/mL Fix cells with PBS containing 4 % paraformaldehyde for 20 minutes at RT and block with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT. Follow by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, wash cells with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.1 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

Target Details for NUMB

Target: NUMB (Numb Homolog (NUMB))

Alternative Name: NUMB

Background: Name/Gene ID: NUMB

Synonyms: NUMB, C14_5527, Numb (Drosophila) homolog, S171, Protein numb homolog, C14orf41, H-Numb, Numb homolog (Drosophila), Protein S171

Gene ID: 8650

UniProt: P49757

Pathways: Cell-Cell Junction Organization