Granulin antibody

Catalog No. ABIN325602

The Mouse Monoclonal anti-Granulin antibody (ABIN325602) specifically detects Granulin in WB, IP and ELISA. The antibody is reactive with Human samples.

Quick Overview for Granulin antibody (ABIN325602)

Target: Granulin (GRN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Granulin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-Granulin Antibody

Specificity: Recognizes human GRN. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rmProgranulin.

Purification: Protein G purified

Immunogen: NS0-derived rhGRN.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IP (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1.0 μg/mL, The detection limit for rhGRN is approximately 2 ng/well. Western Blot: 1-2 μg/mL, Using a colorimetric detection system, the detection limit for rhGRN is ~5 ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25 μg/mL to immunoprecipitate rhGRN from conditioned cell culture medium. The recovered rhGRN can be detected in Western Blots using an affinity-purified polyclonal antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 8.0, 20  mM Tris, 300  mM sodium chloride, 5 % trehalose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for Granulin

Target: Granulin (GRN)

Alternative Name: GRN / Progranulin / Granulin

Background: Name/Gene ID: GRN
Family: Hormone

Synonyms: GRN, Acrogranin, CLN11, Epithelin precursor, GEP, gp88, Granulin, Granulin-epithelin, Granulins, PC cell-derived growth factor, PCDGF, PEPI, PGRN, Proepithelin, Progranulin

Gene ID: 2896

UniProt: P28799