POLR2A/RPB1 antibody (pSer5)

Catalog No. ABIN325784

The Mouse Monoclonal anti-POLR2A/RPB1 antibody is suitable to detect POLR2A/RPB1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IP.

Quick Overview for POLR2A/RPB1 antibody (pSer5) (ABIN325784)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-POLR2A/RPB1 Antibody

Binding Specificity: pSer5

Specificity: Detects endogenous levels of total Rpb1 protein (both phosphorylated and unphosphorylated forms).

Immunogen: Synthetic phosphopeptide (KLH-coupled) containing 10 heptapeptide repeats [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] in which Ser5 is phosphorylated.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IP (1:50), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation. Western Blot 1:1000. Immunoprecipitation 1:50.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol, less than 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A / RNA Polymerase II

Background: Name/Gene ID: POLR2A

Synonyms: POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1

Gene ID: 5430

UniProt: P24928

Pathways: Regulatory RNA Pathways