DYKDDDDK Tag antibody (C-Term)

Catalog No. ABIN329107

This Rabbit Polyclonal antibody specifically detects DYKDDDDK Tag in WB, ELISA and IHC.

Quick Overview for DYKDDDDK Tag antibody (C-Term) (ABIN329107)

Target: DYKDDDDK Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DYKDDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-DYKDDDDK Tag Antibody

Binding Specificity: C-Term

Sequence: DYKDDDDK

Specificity: Recognizes the FLAG epitope tag fused to the amino-terminus of targeted proteins. Reacts with all amino-terminal FLAG tagged proteins so far tested. No reactivity is seen against fusion proteins containing the FLAG epitope tag at the carboxy-terminus. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.

Purification: Immunoaffinity purified

Immunogen: FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the carboxy terminal end to facilitate coupling.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IHC, WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:10000-1:50000. Western Blot: 1:1000-1:5000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Target Type: Tag