MUC1 antibody (FITC)

Catalog No. ABIN334252

Product Details anti-MUC1 Antibody (FITC)

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is conjugated to FITC

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes human Mucin 1. Recognizes the peptide epitope Arg-Pro-Ala-Pro within the protein core of the mucin.

Purification: Protein G purified

Immunogen: Urinary MUC-1 mucin.

Isotype: IgG3

Application Details

Application Notes: Approved: Flo, IF, IHC, IHC-P (1:50)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Flow Cytometry: Neat-1:10. Use 10 μL to label 10^6 cells in 100 μL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: -20°C
Protect from light.

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: EMA / MUC1 (MUC1 Products)

Background: Name/Gene ID: MUC1
Family: Mucin

Synonyms: MUC1, CA15-3, CD227, Carcinoma-associated mucin, CD227 antigen, DF3 antigen, Episialin, Epithelial membrane antigen, H23 antigen, H23AG, Krebs von den Lungen-6, MAM6, MUC1/ZD, MUC-1/SEC, Mucin-1, Pem, KL-6, MUC-1/X, Tumor-associated mucin, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, PUM, EMA, MUC-1, Mucin 1, transmembrane

Gene ID: 4582

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling