NR1H4 antibody (Internal Region)

Catalog No. ABIN334396

This Goat Polyclonal antibody specifically detects NR1H4 in ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for NR1H4 antibody (Internal Region) (ABIN334396)

Target: NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NR1H4 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-NR1H4 Antibody

Binding Specificity: Internal Region

Purpose: Farnesoid X receptor / FXR

Sequence: KSCREKTELT PDQQ

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KSCREKTELTPDQQ, from the internal region of the protein sequence according to NP_005114.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 55 kDa in Human Kidney, Colon, Duodenum and Ileum lysate after 0.5 μg/mL antibody staining (calculated MW of 54.4 kDa according to NP_005114.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NR1H4

Target: NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))

Alternative Name: NR1H4

Background: Farnesoid X receptor, HRR1, NR1H4, nuclear receptor subfamily 1, group H, member 4 , BAR, FXR, HRR-1, RIP14 , farnesol receptor HRR-1

Gene ID: 9971

NCBI Accession: NP_005114

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Carbohydrate Metabolic Process