GADD45G antibody (Internal Region)

Catalog No. ABIN334406

The Goat Polyclonal anti-GADD45G antibody is suitable to detect GADD45G in samples from Human. It has been validated for ELISA, IF and FACS.

Quick Overview for GADD45G antibody (Internal Region) (ABIN334406)

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GADD45G antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-GADD45G Antibody

Binding Specificity: Internal Region

Purpose: GADD45gamma (aa 18 to 28)

Sequence: RMQGAGKALH E

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RMQGAGKALHE, from the internal region of the protein sequence according to NP_006696.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 23 kDa in lysate of cell line A549 and in Human Prostate and Testes lysate at a concentration of 0.5-1 μg/mL (calculated Mwt.of 17.1 kDa according to NP_006696.1). This molecular weight is observed b

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10u

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GADD45G

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Alternative Name: GADD45G

Background: Growth arrest and DNA-damage-inducible, gamma, CR6, DDIT2, GADD45gamma, GRP17, GADD45-gamma, gadd-related protein, 17 kD, growth arrest and DNA-damage-inducible gamma

Gene ID: 10912, 23882, 291005

NCBI Accession: NP_006696

Pathways: Cell Division Cycle