IDE antibody (Internal Region)

Catalog No. ABIN334425

This anti-IDE antibody is a Goat Polyclonal antibody detecting IDE in WB, ELISA and IHC. Suitable for Human.

Quick Overview for IDE antibody (Internal Region) (ABIN334425)

Target: IDE (Insulin-Degrading Enzyme (IDE))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IDE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-IDE Antibody

Binding Specificity: Internal Region

Purpose: Insulysin / Insulinase

Sequence: QQYNFDRDNT E

Specificity: This antibody is expected to recognize all reported isoforms (NP_004960.2, NP_001159418.1, NP_001309722.1, NP_001309723.1, NP_001309724.1, NP_001309726.1). Reported variants represent identical protein: NP_001309725.1, NP_001309724.1

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QQYNFDRDNTE, from the internal region of the protein sequence according to NP_004960.1, NP_001159418.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Lung and Breast. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 110-120 kDa band observed in lysates of cell line K562 (calculated MW of 118 kDa according to NP_004960.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IDE

Target: IDE (Insulin-Degrading Enzyme (IDE))

Alternative Name: IDE

Background: Insulinase, insulysin, insulin-degrading enzyme , FLJ35968, INSULYSIN

Gene ID: 3416

NCBI Accession: NP_004960, NP_001159418, NP_001309722, NP_001309723, NP_001309724, NP_001309726

Pathways: SARS-CoV-2 Protein Interactome