Patched 1 antibody (Internal Region)

Catalog No. ABIN334456

This Goat Polyclonal antibody specifically detects Patched 1 in ELISA and IF. It exhibits reactivity toward Zebrafish (Danio rerio)and has been mentioned in 1 publication.

Quick Overview for Patched 1 antibody (Internal Region) (ABIN334456)

Target: Patched 1 (PTCH1)

Reactivity: Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This Patched 1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-Patched 1 Antibody

Binding Specificity: Internal Region

Purpose: Patched 1 (zebrafish)

Sequence: QTGSKKEPFN YSQ

Cross-Reactivity: Zebrafish (Danio rerio)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QTGSKKEPFNYSQ, from the internal region of the protein sequence according to NP_571063.1.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: This product has been successfully used in IF studies of zebrafish tumors (Ju et al, Mol Cancer. 2009 Jun 25,8:40., PMID: 19555497). Recommended concentration: 1-3μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Patched 1 antibody (ABIN334456)

Ju, Spitsbergen, Eden, Taylor, Chen: "Co-activation of hedgehog and AKT pathways promote tumorigenesis in zebrafish." in: Molecular cancer, Vol. 8, pp. 40, (2009) (PubMed).

Target Details for Patched 1

Target: Patched 1 (PTCH1)

Alternative Name: ptc1

Background: Ptc1, patched 1 , ptc-1, etID309849.2, patched1

NCBI Accession: NP_571063

Pathways: Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation