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Patched 1 antibody (Internal Region)

Verified PTCH1 Reactivity: Zebrafish (Danio rerio) ELISA, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN334456
  • Target See all Patched 1 (PTCH1) Antibodies
    Patched 1 (PTCH1)
    Binding Specificity
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 39
    • 27
    • 9
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Zebrafish (Danio rerio)
    Host
    • 35
    • 7
    • 3
    Goat
    Clonality
    • 39
    • 6
    Polyclonal
    Conjugate
    • 33
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Patched 1 antibody is un-conjugated
    Application
    • 32
    • 23
    • 17
    • 10
    • 6
    • 5
    • 1
    • 1
    ELISA, Immunofluorescence (IF)
    Purpose
    Patched 1 (zebrafish)
    Sequence
    QTGSKKEPFN YSQ
    Cross-Reactivity
    Zebrafish (Danio rerio)
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-QTGSKKEPFNYSQ, from the internal region of the protein sequence according to NP_571063.1.
    Isotype
    IgG
    Top Product
    Discover our top product PTCH1 Primary Antibody
  • Application Notes
    Peptide ELISA: antibody detection limit dilution 1:32000.
    Comment

    Immunofluorescence: This product has been successfully used in IF studies of zebrafish tumors (Ju et al, Mol Cancer. 2009 Jun 25,8:40., PMID: 19555497). Recommended concentration: 1-3μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Ju, Spitsbergen, Eden, Taylor, Chen: "Co-activation of hedgehog and AKT pathways promote tumorigenesis in zebrafish." in: Molecular cancer, Vol. 8, pp. 40, (2009) (PubMed).

  • Target
    Patched 1 (PTCH1)
    Alternative Name
    ptc1 (PTCH1 Products)
    Background
    Ptc1, patched 1 , ptc-1, etID309849.2, patched1
    NCBI Accession
    NP_571063
    Pathways
    Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation
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