PINK1 antibody (Internal Region)

Catalog No. ABIN334460

The Goat Polyclonal anti-PINK1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect PINK1 in samples from Human. There is 1 publication available.

Quick Overview for PINK1 antibody (Internal Region) (ABIN334460)

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PINK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PINK1 Antibody

Binding Specificity: Internal Region

Purpose: PINK1

Sequence: QGKAHLESRS YQEAQ

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QGKAHLESRSYQEAQ, from the internal region of the protein sequence according to NP_115785.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 60-65 kDa band observed in lysates of cell lines Jurkat and HeLa (calculated MW of 62.8 kDa according to NP_115785.1). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PINK1 antibody (ABIN334460)

Choubey, Cagalinec, Liiv, Safiulina, Hickey, Kuum, Liiv, Anwar, Eskelinen, Kaasik: "BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria." in: Autophagy, Vol. 10, Issue 6, pp. 1105-19, (2014) (PubMed).

Target Details for PINK1

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Alternative Name: PINK1

Background: PINK1, PTEN induced putative kinase 1 , BRPK, FLJ27236, PARK6 , protein kinase BRPK, serine/threonine-protein kinase PINK1

Gene ID: 65018, 68943, 298575

NCBI Accession: NP_115785

Pathways: Autophagy