TARDBP antibody (Internal Region)

Catalog No. ABIN334503

This anti-TARDBP antibody is a Goat Polyclonal antibody detecting TARDBP in WB, IHC and ELISA. Suitable for Human.

Quick Overview for TARDBP antibody (Internal Region) (ABIN334503)

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TARDBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-TARDBP Antibody

Binding Specificity: Internal Region

Purpose: TDP-43

Sequence: HISNAEPKHN SNRQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HISNAEPKHNSNRQ, from the internal region of the protein sequence according to NP_031401.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx. 45 kDa band observed in lysates of cell line Jurkat and in Human Cerebellum and approx. 48 kDa in Human Frontal Cortex and Hippocampus lysates (calculated MW of 44.7 kDa according to NP_031401.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TARDBP

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Alternative Name: TARDBP

Background: TARDBP, TAR DNA binding protein, ALS10, TDP-43, OTTHUMP00000002171, TAR DNA-binding protein-43

Gene ID: 23435, 230908

NCBI Accession: NP_031401

Pathways: Positive Regulation of Peptide Hormone Secretion