HNF1A antibody (Internal Region)

Catalog No. ABIN334504

This Goat Polyclonal antibody specifically detects HNF1A in ELISA, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for HNF1A antibody (Internal Region) (ABIN334504)

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HNF1A antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-HNF1A Antibody

Binding Specificity: Internal Region

Purpose: TCF1 / HNF1

Sequence: DELPTKKGRR NRFK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DELPTKKGRRNRFK, from the internal region of the protein sequence according to NP_000536.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: A customer reported a weak band at approx. 75 kDa on lysate of cell line Caco-2, at a concentration of 3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HepG2 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HNF1A

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Alternative Name: HNF1A

Background: TCF1, transcription factor 1, hepatic LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor , HNF1, HNF1A, LFB1, MODY3 , Interferon production regulator factor (HNF1), albumin proximal factor, hepatic nuclear factor 1, maturity onset diabetes of t

Gene ID: 6927, 21405, 24817

NCBI Accession: NP_000536

Pathways: Hormone Transport, Carbohydrate Homeostasis