THRB antibody (Internal Region)

Catalog No. ABIN334509

This anti-THRB antibody is a Goat Polyclonal antibody detecting THRB in WB and ELISA. Suitable for Zebrafish (Danio rerio).

Quick Overview for THRB antibody (Internal Region) (ABIN334509)

Target: THRB (Thyroid Hormone Receptor, beta (THRB))

Reactivity: Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This THRB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-THRB Antibody

Binding Specificity: Internal Region

Purpose: Thrb (zebrafish)

Sequence: DIGSAPIVNA PEGNKVD

Specificity: This antibody is not expected to cross-react to Thra.

Cross-Reactivity: Zebrafish (Danio rerio)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DIGSAPIVNAPEGNKVD, from the internal region of the protein sequence according to NP_571415.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in Zebrafish adult Heart lysates (calculated MW of 44.3 kDa according to NP_571415.1). Recommended concentration: 2-5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for THRB

Target: THRB (Thyroid Hormone Receptor, beta (THRB))

Alternative Name: thrb

Background: Thrb, thyroid hormone receptor beta, NR1A2, TR-beta, TR[b]1, fj24f03, trb, wu:fj24f03

NCBI Accession: NP_571415

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound