Ascorbate Oxidase antibody

Catalog No. ABIN334592

The Rabbit Polyclonal anti-Ascorbate Oxidase antibody is suitable to detect Ascorbate Oxidase in samples from Cucurbita pepo. It has been validated for ELISA, WB and IHC.

Quick Overview for Ascorbate Oxidase antibody (ABIN334592)

Target: Ascorbate Oxidase (AO)

Reactivity: Cucurbita pepo

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ascorbate Oxidase antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Ascorbate Oxidase Antibody

Purpose: Anti-AO | ascorbate oxidase

Cross-Reactivity (Details): Cucurbita sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Glycne max,Oryza sativa

Immunogen: Ascorbate oxidase purified from Cucurbita sp.

Isotype: IgG

Application Details

Application Notes: 1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 0.5 mL of sterile water

Buffer: Total IgG in PBS pH 7.2.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for Ascorbate Oxidase

Target: Ascorbate Oxidase (AO)

Background:

Background: Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90 % of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

Additional information: IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography,

Molecular Weight: 61 | 45 kDa (Cucurbita pepo)