APOB antibody (Internal Region)
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- Target See all APOB Antibodies
- APOB (Apolipoprotein B (APOB))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This APOB antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Purpose
- APOB
- Sequence
- TDLHLRYQKD KK
- Specificity
- This antibody only recognises ApoB-100 and not the cleaved chain ApoB-48.
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-TDLHLRYQKDKK, from the internal region of the protein sequence according to NP_000375.2.
- Isotype
- IgG
- Top Product
- Discover our top product APOB Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 2 μg/mL.
Western Blot: Customer data showed a band corresponding to the exopected size of ApoB-100 in Human serum samples from five different individuals (calculated Mwt of 515 kDa according to NP_000375.3). The smalller bands shown are believed to be degradation
Peptide ELISA: antibody detection limit dilution 1:32000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- APOB (Apolipoprotein B (APOB))
- Alternative Name
- APOB (APOB Products)
- Synonyms
- FLDB antibody, LDLCQ4 antibody, Ac1-060 antibody, Apo B-100 antibody, ApoB-100 antibody, ApoB-48 antibody, AI315052 antibody, apob-100 antibody, apob-48 antibody, APOB-100 antibody, ApoB(100) antibody, APOB antibody, apolipoprotein B antibody, APOB antibody, Apob antibody
- Background
- APOB, apolipoprotein B (including Ag(x) antigen), FLDB, apoB-100, apolipoprotein B,
- Gene ID
- 338
- NCBI Accession
- NP_000375
- Pathways
- Lipid Metabolism
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