APOB antibody (Internal Region)

Catalog No. ABIN335116

This Goat Polyclonal antibody specifically detects APOB in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for APOB antibody (Internal Region) (ABIN335116)

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-APOB Antibody

Binding Specificity: Internal Region

Purpose: APOB

Sequence: TDLHLRYQKD KK

Specificity: This antibody only recognises ApoB-100 and not the cleaved chain ApoB-48.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TDLHLRYQKDKK, from the internal region of the protein sequence according to NP_000375.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 2 μg/mL.

Western Blot: Customer data showed a band corresponding to the exopected size of ApoB-100 in Human serum samples from five different individuals (calculated Mwt of 515 kDa according to NP_000375.3). The smalller bands shown are believed to be degradation

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: APOB

Background: APOB, apolipoprotein B (including Ag(x) antigen), FLDB, apoB-100, apolipoprotein B,

Gene ID: 338

NCBI Accession: NP_000375

Pathways: Lipid Metabolism