MSH5 antibody (Internal Region)

Catalog No. ABIN335148

Product Details anti-MSH5 Antibody

Target: MSH5 (MutS Homolog 5 (MSH5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MSH5 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: MSH5

Sequence: GDLHCEIRDQ ET

Specificity: This antibody is expected to recognise three reported isoforms ( NP_079535.3 , NP_751897.1, NP_002432.1), Reported variants NP_751898.1 and NP_002432.1 represent identical protein.

Predicted Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence GDLHCEIRDQET, from the internal region of the protein sequence according to NP_079535.3 , NP_751897.1, NP_002432.1.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Preliminary experiments gave an approx 38 kDa band in Human Testis, Tonsil and Thymus lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9 kDa according to the smallest reported isoform NP_002432.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide.

DS IHC Results: Paraffin embedded Human Testis and Thymus. Recommended concentration: 2.5-3.75 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MSH5

Target: MSH5 (MutS Homolog 5 (MSH5))

Alternative Name: MSH5 (MSH5 Products)

Synonyms: MSH5 antibody, G7 antibody, MUTSH5 antibody, NG23 antibody, Mut5 antibody, mutS homolog 5 antibody, mutS protein homolog 5 antibody, MSH (MutS Homolog) family antibody, MSH5 antibody, msh5 antibody, LOC100635155 antibody, Msh5 antibody, msh-5 antibody

Background: MSH5, mutS homolog 5 (E. coli) , XXbac-BCX40G17.5, DKFZp434C1615, G7N: MGC2939, MutSH5, NG23, mutS homolog 5

Gene ID: 4439

NCBI Accession: NP_079535, NP_751897, NP_002432

Pathways: M Phase