MSH5 antibody (Internal Region)

Catalog No. ABIN335148

Product Details anti-MSH5 Antibody

Target: MSH5 (MutS Homolog 5 (MSH5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MSH5 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: MSH5

Sequence: GDLHCEIRDQ ET

Specificity: This antibody is expected to recognise three reported isoforms ( NP_079535.3 , NP_751897.1, NP_002432.1), Reported variants NP_751898.1 and NP_002432.1 represent identical protein.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence GDLHCEIRDQET, from the internal region of the protein sequence according to NP_079535.3 , NP_751897.1, NP_002432.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis and Thymus. Recommended concentration: 2.5-3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 38 kDa band in Human Testis, Tonsil and Thymus lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calc

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MSH5

Target: MSH5 (MutS Homolog 5 (MSH5))

Alternative Name: MSH5 (MSH5 Products)

Background: MSH5, mutS homolog 5 (E. coli) , XXbac-BCX40G17.5, DKFZp434C1615, G7N: MGC2939, MutSH5, NG23, mutS homolog 5

Gene ID: 4439, 17687, 294252

NCBI Accession: NP_079535, NP_751897, NP_002432

Pathways: M Phase