Vimentin antibody

Catalog No. ABIN335381

The Mouse Monoclonal anti-Vimentin antibody has been validated for WB, IHC, FACS, ICC and IHC (fro). It is suitable to detect Vimentin in samples from Human, Rat, Pig, Dog, Goat and Hamster. There is 1 publication available.

Quick Overview for Vimentin antibody (ABIN335381)

Target: Vimentin (VIM)

Reactivity: Human, Rat, Pig, Dog, Goat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RV203

Product Details anti-Vimentin Antibody

Specificity: Human, rat, goat, canine, hamster and swine.

Purification: Purified

Immunogen: RV203 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Isotype: IgG1

Application Details

Application Notes: RV203 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV203 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-Vimentin antibody (ABIN335381)

Schaart, Pieper, Kuijpers, Bloemendal, Ramaekers: "Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins." in: Differentiation; research in biological diversity, Vol. 46, Issue 2, pp. 105-15, (1991) (PubMed).

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Pathways: Caspase Cascade in Apoptosis