Keratin 10/13 antibody

Catalog No. ABIN336868

This Mouse Monoclonal antibody specifically detects Keratin 10/13 in IHC. It exhibits reactivity toward Human.

Quick Overview for Keratin 10/13 antibody (ABIN336868)

Target: Keratin 10/13

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Clone: CK208

Product Details

Specificity: Molecular Weight: CK 10 is 56.9 kDa and CK 13 is 53 kDa.

Immunogen: Cytokeratin extraction from human ectocervical epithelium

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry (IHC), Immunocytochemistry (ICC) (frozen tissue sections ONLY or cell smears) For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. The optimum dilution should be determined by the individual lab. Positive Control: Human tonsil or skin. Cellular Localization: Cytoplasmic.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The concentrated antibodies are in supplied in PBS with 1% BSA, 0.05% azide, pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: Keratin 10/13

Alternative Name: Cytokeratin 10+13

Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20. This antibody reacts with 53 kDa and 56.6 kDa cytokeratin (CK) proteins by Western blot. With frozen sections, this antibody serves as a differentiation related marker for all stratified epithelia and stains all suprabasal cell in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. Recently Silveira et. al. (3) have reported that in tongue squamous cell carcinoma the expression of CK 10 was significantly correlated with the presence of metasis.