p53 antibody

Catalog No. ABIN336891

This anti-p53 antibody is a Mouse Monoclonal antibody detecting p53 in IHC and IHC (p). Suitable for Human.

Quick Overview for p53 antibody (ABIN336891)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: P53-221

Product Details anti-p53 Antibody

Specificity: Molecular Weight: 53 kDa.

Immunogen: Recombinant human wild type p53 protein

Isotype: IgG2a

Application Details

Application Notes: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. FFPE tissue section requires antigen retriever (boiling tissue in 10 mM citrate, pH 6.0 for 20 mins, followed by cooling at RT for 20 mins). IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Human colon Cancer. Cellular Localization: Nuclear.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibodies are supplied with 1% BSA in PBS with 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description p53, also known as protein 53 (TP53), is a transcription factor th

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect