PARD6A antibody (AA 334-346)

Catalog No. ABIN337017

The Goat Polyclonal anti-PARD6A antibody (ABIN337017) specifically detects PARD6A in ELISA, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for PARD6A antibody (AA 334-346) (ABIN337017)

Target: PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PARD6A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PARD6A Antibody

Binding Specificity: AA 334-346

Specificity: Human PARD6A / PAR6. This antibody is expected to recognise both reported isoforms (NP_058644.1, NP_001032358.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Marmoset, Bovine, Elephant (92%) Gibbon, Dog, Bat, Panda, Rabbit, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-GSRIRGDGSGFSL from the C-terminus of human PARD6A / PAR6 (NP_058644.1, NP_001032358.1). Percent identity by BLAST analysis: Human, Gorilla (100%), Marmoset, Bovine, Elephant (92%), Gibbon, Dog, Bat, Panda, Rabbit, Horse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:8000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PARD6A

Target: PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))

Alternative Name: PARD6A / PAR6

Background: Name/Gene ID: PARD6A

Synonyms: PARD6A, PAR-6 alpha, PAR6A, Tax interaction protein 40, Tax-interacting protein 40, PAR-6, PAR-6A, PAR6C, TIP-40, PAR6, PAR6alpha, TAX40

Gene ID: 50855

NCBI Accession: NP_058644, NP_001032358

UniProt: Q9NPB6

Pathways: Cell-Cell Junction Organization