Isocitrate Dehydrogenase antibody

Catalog No. ABIN337057

This Rabbit Polyclonal antibody specifically detects Isocitrate Dehydrogenase in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Pig and Vicia faba.

Quick Overview for Isocitrate Dehydrogenase antibody (ABIN337057)

Target: Isocitrate Dehydrogenase (IDH)

Reactivity: Human, Pig, Vicia faba

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Isocitrate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Isocitrate Dehydrogenase Antibody

Specificity: Binds both Dehydroepiandrosterone (DHEA) and DHEAS, demonstrating minimal cross-reactivity with Progesterone and Testosterone. DHEA is the most abundant hormone in the human body and a precursor of all the sex hormones. It is synthesized principally in the adrenal glands and gonads from cholesterol, through conversion to pregnenolone and 17a-Hydroxypregnenolone, by the action of P450 enzymes. DHEA is mainly found in serum as the sulphated form (DHEAS), a reversible reaction catalyzed by sulfotransferase in the adrenal glands, liver and small intestine.

Purification: Affinity purified

Immunogen: Purified isocitrate dehydrogenase from porcine heart.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:400 - 1:1600), IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Target Details for Isocitrate Dehydrogenase

Target: Isocitrate Dehydrogenase (IDH)