KDM2B antibody (AA 1314-1326)

Catalog No. ABIN337061

The Goat Polyclonal anti-KDM2B antibody is suitable to detect KDM2B in samples from Human, Mouse, Dog, Rat, Cow, Horse, Rabbit, Chicken, Bat, Hamster and Monkey. It has been validated for ELISA, IHC and IHC (p).

Quick Overview for KDM2B antibody (AA 1314-1326) (ABIN337061)

Target: KDM2B (Lysine (K)-Specific Demethylase 2B (KDM2B))

Reactivity: Human, Mouse, Dog, Rat, Cow, Horse, Rabbit, Chicken, Bat, Hamster, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This KDM2B antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KDM2B Antibody

Binding Specificity: AA 1314-1326

Specificity: Human KDM2B / FBXL10. This antibody is expected to recognize isoform a (NP_115979.3) only.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Turkey, Chicken, Platypus (100%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-FGQVEEKLLQKLS from the C-terminus of human KDM2B / FBXL10 (NP_115979.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Turkey, Chicken, Platypus (100%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for KDM2B

Target: KDM2B (Lysine (K)-Specific Demethylase 2B (KDM2B))

Alternative Name: KDM2B / FBXL10

Background: Name/Gene ID: KDM2B
Family: F-Box

Synonyms: KDM2B, CXXC2, Fbl10, F-box/LRR-repeat protein 10, JHDM1B, F-box protein FBL10, FBXL10, Lysine-specific demethylase 2B, PCCX2, Protein JEMMA

Gene ID: 84678

NCBI Accession: NP_115979

UniProt: Q8NHM5

Pathways: Tube Formation, Warburg Effect