NOTCH3 antibody (AA 2306-2318)

Catalog No. ABIN337107

The Goat Polyclonal anti-NOTCH3 antibody (ABIN337107) specifically detects NOTCH3 in ELISA, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Monkey and Bat samples.

Quick Overview for NOTCH3 antibody (AA 2306-2318) (ABIN337107)

Target: NOTCH3 (Notch 3 (NOTCH3))

Reactivity: Human, Mouse, Rat, Monkey, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This NOTCH3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NOTCH3 Antibody

Binding Specificity: AA 2306-2318

Specificity: Human NOTCH3.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Panda, Bat (100%) Hamster, Bovine (92%) Elephant (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QLGPQPEVTPKRQ from the C-terminus of human NOTCH3 (NP_000426.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Panda, Bat (100%), Hamster, Bovine (92%), Elephant (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NOTCH3

Target: NOTCH3 (Notch 3 (NOTCH3))

Alternative Name: NOTCH3

Background: Name/Gene ID: NOTCH3
Family: Fibronectin

Synonyms: NOTCH3, CASIL, CADASIL, Notch 3, Notch (Drosophila) homolog 3, Notch homolog 3, Notch homolog 3 (Drosophila)

Gene ID: 4854

NCBI Accession: NP_000426

UniProt: Q9UM47

Pathways: Notch Signaling