ORAI3 antibody (N-Term)

Catalog No. ABIN337113

The Rabbit Polyclonal anti-ORAI3 antibody (ABIN337113) specifically detects ORAI3 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ORAI3 antibody (N-Term) (ABIN337113)

Target: ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORAI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ORAI3 Antibody

Binding Specificity: N-Term

Specificity: Recognizes human ORAI3, also known as Transmembrane protein 142C (TMEM142C), a 31.5 kD multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Studies indicate that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. AHP1496 is not expected to cross react with either ORAI1 or ORAI2.

Purification: Affinity purified

Immunogen: A 15 amino acid peptide from near the amino terminus of human ORAI3.

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: LS-B2385 detects a band of approximately 35kD in A20 cell lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Target Details for ORAI3

Target: ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))

Alternative Name: ORAI3

Background: Name/Gene ID: ORAI3

Synonyms: ORAI3, Protein orai-3, TMEM142C, Transmembrane protein 142C

Gene ID: 93129

UniProt: Q9BRQ5