Osteopontin antibody (AA 288-299)

Catalog No. ABIN337138

This Goat Polyclonal antibody specifically detects Osteopontin in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Osteopontin antibody (AA 288-299) (ABIN337138)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Osteopontin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Osteopontin Antibody

Binding Specificity: AA 288-299

Specificity: Human SPP1 / Osteopontin.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) Marmoset (92%) Mouse, Panda, Dog, Pig, Arthrobotrys (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DPKSKEEDKHLK from the C-terminus of human SPP1 / Osteopontin (NP_001035147.1, NP_000573.1, NP_001035149.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%), Marmoset (92%), Mouse, Panda, Dog, Pig, Arthrobotrys (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: SPP1 / Osteopontin

Background: Name/Gene ID: SPP1

Synonyms: SPP1, BSPI, BNSP, Bone sialoprotein 1, ETA-1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin, Osteopontin, Bone sialoprotein I

Gene ID: 6696

NCBI Accession: NP_001035147, NP_000573, NP_001035149

UniProt: P10451

Pathways: Regulation of Cell Size