PRMT5 antibody

Catalog No. ABIN337159

This Rabbit Polyclonal antibody specifically detects PRMT5 in WB, IHC, IHC (p) and IP. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for PRMT5 antibody (ABIN337159)

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRMT5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-PRMT5 Antibody

Specificity: Recognizes endogenous levels of total human Skb1Hs methyltransferase protein at 70kD. Does not cross-react with other related mammalian methyltransferases. Species cross-reactivity: monkey, mouse and rat.

Purification: Protein A purified

Immunogen: Synthetic peptide (KLH coupled) corresponding to residues surrounding amino acid 100 of human Skb1Hs methyltransferase.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100), IP (1:100), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for PRMT5

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Alternative Name: PRMT5

Background: Name/Gene ID: PRMT5

Synonyms: PRMT5, 72 kDa ICln-binding protein, HRMT1L5, JBP1, Jak-binding protein 1, IBP72, SKB1Hs, SKB1, SKB1 homolog, Skb1 (S. pombe) homolog, SKB1 homolog (S. pombe)

Gene ID: 10419

UniProt: O14744

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development