APOA2 antibody

Catalog No. ABIN337192

This Goat Polyclonal antibody specifically detects APOA2 in WB, IHC, ELISA, IP and IHC (p). It exhibits reactivity toward Human.

Quick Overview for APOA2 antibody (ABIN337192)

Target: APOA2 (Apolipoprotein A-II (APOA2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APOA2 Antibody

Specificity: Recognizes human apoLipoprotein A-II. Exhibits very low cross-reactivity with Type A-I, B, C-I, C-II, C-III, E and J apoLipoproteins or other human serum proteins.

Purification: Immunoaffinity purified

Immunogen: Highly purified apoLipoprotein Type A-II from human plasma.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:2500 - 1:5000), IHC, IHC-P (5 μg/mL), IP, WB (1:2500 - 1:5000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA,  pH 8.0, 0.01 % sodium azide, before the addition of glycerol to 40 %. No stabilizing proteins added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for APOA2

Target: APOA2 (Apolipoprotein A-II (APOA2))

Alternative Name: APOA2 / Apolipoprotein A II

Background: Name/Gene ID: APOA2

Synonyms: APOA2, Apo-AII, ApoAII, ApoA-II, Apolipoprotein A-II, Apolipoprotein A2, Apolipoprotein A II

Gene ID: 336

UniProt: P02652

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Negative Regulation of Transporter Activity, Lipid Metabolism