NACA antibody (C-Term)

Catalog No. ABIN337196

The Rabbit Polyclonal anti-NACA antibody has been validated for WB, IHC and IHC (p). It is suitable to detect NACA in samples from Human, Mouse and Rat.

Quick Overview for NACA antibody (C-Term) (ABIN337196)

Target: NACA (NACa1) (Nascent Polypeptide Associated Complex alpha 1 (NACa1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NACA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NACA Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Recognizes the human alpha-NAC protein. On Western blots, it identifies a single band at ~23kD. Species cross-reactivity: mouse and rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of the human alpha-NAC protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: Fetal mouse brain, rat brain homogenates and human NGP96 cell lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NACA

Target: NACA (NACa1) (Nascent Polypeptide Associated Complex alpha 1 (NACa1))

Alternative Name: NACA

Background: Name/Gene ID: NACA

Synonyms: NACA, Allergen Hom s 2, Alpha-NAC, HSD48, NAC-alpha, NACA1

Gene ID: 4666

UniProt: Q13765

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development