NACA antibody (pSer43)

Catalog No. ABIN7222642

The Rabbit Polyclonal anti-NACA antibody (ABIN7222642) specifically detects NACA in IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NACA antibody (pSer43) (ABIN7222642)

Target: NACA (NACa1) (Nascent Polypeptide Associated Complex alpha 1 (NACa1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NACA antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-NACA Antibody

Binding Specificity: pSer43

Purpose: Rabbit Anti-NACA (phospho Ser43) Polyclonal Antibody

Specificity: Phospho-NACA (S43) Polyclonal Antibody detects endogenous levels of NACA protein only when phosphorylated at S43.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human NACA Phospho-Ser43

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NACA

Target: NACA (NACa1) (Nascent Polypeptide Associated Complex alpha 1 (NACa1))

Alternative Name: NACA

Background: NACA, HSD48, Nascent polypeptide-associated complex subunit alpha, NAC-alpha, Alpha-NAC, allergen Hom s 2NACA encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of NACA on different chromosomes.

Gene ID: 4666

UniProt: Q13765

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development