IRF3 antibody

Catalog No. ABIN337252

This Rabbit Polyclonal antibody specifically detects IRF3 in WB, IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for IRF3 antibody (ABIN337252)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IRF3 Antibody

Specificity: Detects endogenous levels of human IRF3 at 51kD mouse and rat, at 57kD human. Species cross-reactivity: mouse, rat and monkey.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (KLH-coupled) corresponding to a region around residue 300 of IRF3.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:50), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: IRF3 / IRF-3

Background: Name/Gene ID: IRF3

Synonyms: IRF3, Interferon regulatory factor 3, IRF-3

Gene ID: 3661

UniProt: Q14653

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades