ATF6 antibody (AA 1-273)

Catalog No. ABIN337286

The Mouse Monoclonal anti-ATF6 antibody has been validated for WB, IHC, IHC (p), ICC and ChIP. It is suitable to detect ATF6 in samples from Human, Mouse, Rat and Hamster.

Quick Overview for ATF6 antibody (AA 1-273) (ABIN337286)

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Reactivity: Human, Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Clone: 70B1413-1

Product Details anti-ATF6 Antibody

Binding Specificity: AA 1-273

Specificity: A partial protein containing amino acids 1-273 of human ATF6.

Purification: Protein G purified

Immunogen: Recombinant human ATF6.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ChrIP, ICC, IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: TBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Alternative Name: ATF6

Background: Name/Gene ID: ATF6

Synonyms: ATF6, ATF6-alpha, ATF6A

Gene ID: 22926

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response