VWF antibody
Quick Overview for VWF antibody (ABIN337312)
Target
See all VWF AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- Recognizes human vWF.
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Purification
- Caprylic acid and ammonium sulfate precipitations, followed by Protein G affinity chromatography
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Immunogen
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Human von Willebrand Factor (vWF) purified from plasma.
Type of Immunogen: Purified protein -
Isotype
- IgG
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Application Notes
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Approved: ELISA, IE, IHC, IHC-P (5 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 10 mM HEPES, 0.15 M sodium chloride, pH 7.4, 50 % glycerol.
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- VWF (Von Willebrand Factor (VWF))
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Alternative Name
- VWF / Von Willebrand Factor
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Background
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Name/Gene ID: VWF
Synonyms: VWF, Coagulation factor VIII VWF, F8VWF, VWD, Von Willebrand factor -
Gene ID
- 7450
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UniProt
- P04275
Target
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