IGF1R antibody

Catalog No. ABIN337334

The Mouse Monoclonal anti-IGF1R antibody is suitable to detect IGF1R in samples from Human. It has been validated for WB, IHC, FACS and IHC (p).

Quick Overview for IGF1R antibody (ABIN337334)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGF1R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1H7

Product Details anti-IGF1R Antibody

Specificity: Recognizes human CD221, a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Clone 1H7 is reported to partially block binding of IGF-I and IGF-II to CD221.

Purification: Protein G purified

Immunogen:

Type of Immunogen: Native protein

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:10), IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Histology Positive Control Tissue: Pancreas, placenta.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: IGF1R / IGF1 Receptor

Background: Name/Gene ID: IGF1R
Subfamily: Insulin receptor
Family: Protein Kinase

Synonyms: IGF1R, CD221 antigen, CD221, IGF1 Receptor, IGFR, IGF-I receptor, Soluble IGF1R variant 1, Soluble IGF1R variant 2, Type 1 IGF receptor, IGFIR, JTK13

Gene ID: 3480

UniProt: P08069

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy