Complement C2 antibody

Catalog No. ABIN337358

Product Details anti-Complement C2 Antibody

Target: Complement C2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complement C2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes C2 from human plasma/serum. Epitope specificity differs from that of LS-C22277 and LS-C22278 as determined by inhibition ELISA. Reacts strongly with C2. Strong reaction is seen with human serum/plasma and no reaction is seen with serum from patients deficient in C2, when tested in sandwich ELISA with a polyclonal antibody against C2. In ELISA, reaction is also seen with C2 directly coated onto the microtiter well.

Purification: Protein A/G purified

Immunogen: Complement component C2 isolated from human plasma.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:2000), IHC, IHC-P (10 μg/mL), WB (1:125)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: After SDS-PAGE, this antibody reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Complement C2

Target: Complement C2

Abstract: Complement C2 Products

Synonyms: CO2 antibody, complement C2 antibody, complement component 2 (within H-2S) antibody, C2 antibody

Background: Name/Gene ID: C2
Subfamily: Serine S1
Family: Protease

Synonyms: C2, C2b, C3/C5 convertase, Complement component C2, Complement C2, Complement component 2, CO2

Gene ID: 717

UniProt: P06681