HSF1 antibody
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- Target See all HSF1 Antibodies
- HSF1 (Heat Shock Factor Protein 1 (HSF1))
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Reactivity
- Human, Mouse, Rat, Rabbit, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This HSF1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Recognizes human HSF-1 at ~85kD protein in unstressed cells and an ~95kD protein in heat shocked cells, corresponding to the molecular masses of the inactive and activated forms respectively of HSF-1. Species cross-reactivity: mouse, rat, monkey, rabbit.
- Purification
- Protein A purified
- Immunogen
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Recombinant human HSF-1 expressed in E. coli.
Type of Immunogen: Recombinant protein - Isotype
- IgG
- Top Product
- Discover our top product HSF1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (1:500)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Positive control: Recombinant Human HSF-1 Protein. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.09 % sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C,-20 °C
- Storage Comment
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Target
- HSF1 (Heat Shock Factor Protein 1 (HSF1))
- Alternative Name
- HSF1 (HSF1 Products)
- Synonyms
- hsf1 antibody, Hsf antibody, xhsf1 antibody, AA960185 antibody, HSTF1 antibody, HSF1 antibody, HSF antibody, HSF1c antibody, hsf1a antibody, hsf1b antibody, zHSF1a antibody, zHSF1b antibody, hstf1 antibody, heat shock factor antibody, Heat Shock Factor antibody, heat shock factor 1 antibody, heat shock transcription factor 1 antibody, heat shock transcription factor 1 S homeolog antibody, hsf1 antibody, hsf-1 antibody, Hsf1 antibody, HSF1 antibody, hsf1.S antibody
- Background
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Name/Gene ID: HSF1
Synonyms: HSF1, HSTF 1, Heat shock factor protein 1, HSF 1, HSTF1 - Gene ID
- 3297
- UniProt
- Q00613
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