Luciferase antibody

Catalog No. ABIN339711

This anti-Luciferase antibody is a Mouse Monoclonal antibody detecting Luciferase in WB, IF, IHC and IHC (fro). Suitable for Photinus pyralis.

Quick Overview for Luciferase antibody (ABIN339711)

Target: Luciferase

Reactivity: Photinus pyralis

Host: Mouse

Clonality: Monoclonal

Conjugate: This Luciferase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Luciferase Antibody

Specificity: This antibody specifically reacts with luciferase protein, a reporter used to quantify expression levels from promoters in tissue culture and in vivo. Reactivity was confirmed via Western Blot analysis on the following samples containing added purified luciferase protein: mouse heart, liver, kidney, and cortex tissues. There was no cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts. In IHC (frozen tissue) and Immunofluorescence applications, our antibody was able to detect tissue specific expression of Luciferase protein in Drosophila embryos using the well-characterized Gal4-UAS system with two different Gal4 drivers.

Purification: Protein A purified

Immunogen: Luciferase protein from Photinus pyralis.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: IF (10 - 30 μg/mL), IHC, IHC-Fr, WB (1 - 3 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Western Blot: 1-3 μg/mL. Observed in mouse heart, liver, kidney, and cortex tissues containing added purified luciferase protein. Immunohistochemistry (Frozen Tissue): 15-50 μg/mL. Detects tissue specific expression of Luciferase protein in Drosophila embryos using Gal4-UAS system with two different Gal4 drivers. Immunofluorescence: 10-30 μg/mL.

Comment:

Target Species of Antibody: Photinus pyralis

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Luciferase

Target: Luciferase

Alternative Name: Firefly Luciferase

Background: Synonyms: Firefly Luciferase, PPLUC

UniProt: P08659