CD14 antibody

Catalog No. ABIN343172

The Mouse Monoclonal anti-CD14 antibody has been validated for FACS, WB, ELISA and Func. It is suitable to detect CD14 in samples from Human. There are 4+ publications available.

Quick Overview for CD14 antibody (ABIN343172)

Target: CD14

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Functional Studies (Func)

Clone: BiG 7

Product Details anti-CD14 Antibody

Purification: Protein G purified

Immunogen: Monocytes and immunoaffinity purified soluble CD14

Isotype: IgG1

Application Details

Application Notes: Suitable for immunostaining of CD14 positive cells, (Flow cytometry), ELISA, Suitable for Western blot (non reducing). Non inhibition of LPS binding to CD14, binding epitope: amino acid 9-13, 39-44, Binding titre: 1:10.000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1 mg/ml

Buffer: 0.1 mg/100 ul PBS without NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-CD14 antibody (ABIN343172)

Muansson, Kjaell, Pellett, Nagy, Welch, Baeckhed, Frisan, Richter-Dahlfors: "Role of the lipopolysaccharide-CD14 complex for the activity of hemolysin from uropathogenic Escherichia coli." in: Infection and immunity, Vol. 75, Issue 2, pp. 997-1004, (2007) (PubMed).

Neilsen, Zimmerman, McIntyre: "Escherichia coli Braun lipoprotein induces a lipopolysaccharide-like endotoxic response from primary human endothelial cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 9, pp. 5231-9, (2001) (PubMed).

Fan, Stelter, Menzel, Jack, Spreitzer, Hartung, Schuett: "Structures in Bacillus subtilis are recognized by CD14 in a lipopolysaccharide binding protein-dependent reaction." in: Infection and immunity, Vol. 67, Issue 6, pp. 2964-8, (1999) (PubMed).

Dziarski, Tapping, Tobias: "Binding of bacterial peptidoglycan to CD14." in: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8680-90, (1998) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: BiG 7 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades