CD14 antibody

Catalog No. ABIN343179

The Mouse Monoclonal anti-CD14 antibody (Clone biG57) (ABIN343179) specifically detects CD14 in ELISA. The antibody is reactive with Mouse samples.

Quick Overview for CD14 antibody (ABIN343179)

Target: CD14

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: ELISA

Clone: BiG57

Product Details anti-CD14 Antibody

Specificity: Recombinant mouse CD14. The antibody don't blocks LPS binding to membrane bound CD14.

Purification: Protein G affinity column

Immunogen: recombinant mouse CD14

Isotype: IgG1

Application Details

Application Notes: ELISA , immunostaining of mouse macrophages after stimulation 6h with LPS, Western blot with recombinant mouse CD14. Binding titre at mouse CD14 transfected CHO-cells: more than 100.000 (ELISA, Flow cytometry), no binding at LPS-binding site, endotoxin content less than 0.1ng/ml

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with bidistilled water

Concentration: 1 mg/mL

Buffer: PBS, pH 7.15

Preservative: Without preservative

Storage: -20 °C,-80 °C

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: Background: biG 57 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Molecular Weight: ~50kDa

Gene ID: 12475

NCBI Accession: NP_033971

UniProt: P10810

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades