Collagen IV antibody

Catalog No. ABIN344800

This Goat Polyclonal antibody specifically detects Collagen IV in ELISA, IHC, IHC (fro) and ICC. It exhibits reactivity toward Human.

Quick Overview for Collagen IV antibody (ABIN344800)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-Collagen IV Antibody

Specificity: Reacts with conformational determinants on human type IV collagen. Less than 10 % cross-reactivity with collagen types I, II, III, V and VI. May show reactivity to type IV collagen from other species. Has not been tested with other extracellular matrix proteins (e. g., laminin, fibronectin). Species cross-reactivity: Bovine.

Purification: Affinity purified

Immunogen: Human and bovine placental collagen type IV.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, IHC, IHC-Fr

Usage: Suitable for use in Dot and Slot Blot, ELISA, Immunohistochemistry (frozen) and Immunocytochemistry. Not suitable for use in Western Blot and Immunohistochemistry (paraffin). Dot and slot blot: 1:100-1:500. ELISA: 1:1000-1:4000. Immunohistochemistry (Indirect): 1:10-1:40 (frozen sections only). Immunocytochemistry: 1:10-1:20. Positive control: Kidney, muscle, tendon, spleen tissues.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM borate buffered saline,  pH 8.0.

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen IV