Huntingtin antibody (AA 1150-1200)

Catalog No. ABIN350377

This Rabbit Polyclonal antibody specifically detects Huntingtin in IHC and WB. It exhibits reactivity toward Rat.

Quick Overview for Huntingtin antibody (AA 1150-1200) (ABIN350377)

Target: Huntingtin (HTT)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Huntingtin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-Huntingtin Antibody

Binding Specificity: AA 1150-1200

Purpose: Rabbit antibody to Huntingtin

Specificity: Specific for Huntingtin.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 1150-1200 of rat Huntingtin conjugated to blue carrier protein was used as the antigen. The peptide shares 87% identity with mouse and human sequences.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Huntingtin

Target: Huntingtin (HTT)

Alternative Name: Huntingtin

Background: FUNCTION: May play a role in microtubule-mediated transport or vesicle function. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. TISSUE SPECIFICITY: Expressed to a high degree in all the regions of the brain of adults and in meiotic cells of the testis. In addition very low levels are detected in various non-neuronal tissues (heart muscle liver lung and kidney). DEVELOPMENTAL STAGE: Identified at high levels in neuronal tissues of embryos as early as day 14.5. This expression remains constant in all further development stages (up to the adult). On the other hand the expression in non-neuronal tissues is down-regulated from stage 17.5 day old embryos.

UniProt: P51111

Pathways: PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport