TRKB antibody (Phosphospecific)

Catalog No. ABIN351082

This Sheep Polyclonal antibody specifically detects TRKB in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for TRKB antibody (Phosphospecific) (ABIN351082)

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This TRKB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TRKB Antibody

Binding Specificity: AA 730-821, Phosphospecific

Purpose: Sheep antibody to phospho Y816 TrkB

Specificity: Specific for TrkB.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 730-821 (including phospho Y816 site) of rat TrkB conjugated to ablue carrier protein was used as the antigen. The peptide is homologous in human and mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. This antibody is also likely to recognise the unphosphorylated form.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for TRKB

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Alternative Name: TrkB

Background: FUNCTION: Receptor for brain-derived neurotrophic factor (BDNF) neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1 PI-3 kinase and PLC-gamma-1. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 and ARMS. SUBCELLULAR LOCATION: Membrane, Single-pass type I membrane protein.

UniProt: Q16620

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport