Annexin A11 antibody (AA 8-20)

Catalog No. ABIN351460

This Goat Polyclonal antibody specifically detects Annexin A11 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Rabbit and Monkey.

Quick Overview for Annexin A11 antibody (AA 8-20) (ABIN351460)

Target: Annexin A11 (ANXA11)

Reactivity: Human, Rabbit, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Annexin A11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Annexin A11 Antibody

Binding Specificity: AA 8-20

Specificity: Human ANXA11 / Annexin A11. NP_001148.1, NP_665875.1 and NP_665876.1 are varients that encode the same protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rabbit (100%) Mouse, Rat, Hamster, Elephant, Dog, Bovine, Pig, Platypus (92%) Monkey, Horse, Opossum, Salmon, Stickleback, Pufferfish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide YPGYPPPPGGYPP-C from the N-terminus of human ANXA11 / Annexin A11 (NP_001148.1, NP_665875.1, NP_665876.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rabbit (100%), Mouse, Rat, Hamster, Elephant, Dog, Bovine, Pig, Platypus (92%), Monkey, Horse, Opossum, Salmon, Stickleback, Pufferfish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Annexin A11

Target: Annexin A11 (ANXA11)

Alternative Name: ANXA11 / Annexin XI

Background: Name/Gene ID: ANXA11

Synonyms: ANXA11, 56 kDa autoantigen, Annexin A11, Annexin XI, CAP-50, Annexin-11, ANX11, Autoantigen, 56-kD, CAP50

Gene ID: 311

NCBI Accession: NP_001148, NP_665875, NP_665876

UniProt: P50995