Axin antibody (AA 850-862)

Catalog No. ABIN351463

This Goat Polyclonal antibody specifically detects Axin in ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Monkey, Cow, Bat, Hamster, Horse and Zebrafish (Danio rerio).

Quick Overview for Axin antibody (AA 850-862) (ABIN351463)

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Reactivity: Human, Mouse, Rat, Monkey, Cow, Bat, Hamster, Horse, Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This Axin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Axin Antibody

Binding Specificity: AA 850-862

Specificity: Human AXIN1. This antibody is expected to recognise both reported isoforms.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Horse, Opossum, Stickleback, Pufferfish, Zebrafish (100%) Turkey, Chicken (92%) Xenopus, Sea anemone (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-FEEKIIGKVEKVD from the C-terminus of human AXIN1 (NP_003493.1, NP_851393.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Horse, Opossum, Stickleback, Pufferfish, Zebrafish (100%), Turkey, Chicken (92%), Xenopus, Sea anemone (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:128000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Axin

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Alternative Name: AXIN1 / Axin-1

Background: Name/Gene ID: AXIN1

Synonyms: AXIN1, Axis inhibitor 1, AXIN, Axin 1, Axis inhibition protein 1, Fused, mouse, homolog of, HAxin, PPP1R49, Axin-1

Gene ID: 8312

NCBI Accession: NP_003493, NP_851393

UniProt: O15169

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling