Amphiphysin antibody (AA 159-170)

Catalog No. ABIN351482

The Goat Polyclonal anti-Amphiphysin antibody is suitable to detect Amphiphysin in samples from Human, Mouse, Rat, Cow, Monkey, Dog, Horse, Rabbit, Hamster and Bat. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for Amphiphysin antibody (AA 159-170) (ABIN351482)

Target: Amphiphysin (AMPH)

Reactivity: Human, Mouse, Rat, Cow, Monkey, Dog, Horse, Rabbit, Hamster, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This Amphiphysin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Amphiphysin Antibody

Binding Specificity: AA 159-170

Specificity: Human AMPH / Amphiphysin. This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%) Xenopus (92%) Opossum, Chicken, Lizard (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KRKDESRISKAE from an internal region of human AMPH / Amphiphysin (NP_001626.1, NP_647477.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%), Xenopus (92%), Opossum, Chicken, Lizard (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (0.1 - 0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Amphiphysin

Target: Amphiphysin (AMPH)

Alternative Name: AMPH / Amphiphysin

Background: Name/Gene ID: AMPH

Synonyms: AMPH, AMPH1, Amphiphysin, Amphiphysin I

Gene ID: 273

NCBI Accession: NP_001626, NP_647477

UniProt: P49418