GLI2 antibody (AA 46-60)

Catalog No. ABIN351530

The Rabbit Polyclonal anti-GLI2 antibody is suitable to detect GLI2 in samples from Human. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for GLI2 antibody (AA 46-60) (ABIN351530)

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GLI2 Antibody

Binding Specificity: AA 46-60

Specificity: Amino acids 46-60 of human Gli-2 (isoform a).

Purification: Immunoaffinity purified

Immunogen: Corresponds to amino acids 46-60 of human Gli-2 (isoform a).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:12000), IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Multiple splice variants have been reported for this protein. Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Alternative Name: GLI2

Background: Name/Gene ID: GLI2

Synonyms: GLI2, GLI-Kruppel family member GLI2, HPE9, Gli-2, Oncogene GLI2, Tax helper protein 2, THP, Zinc finger protein GLI2, Tax helper protein, THP2, GLI family zinc finger 2, Tax helper protein 1, THP1

Gene ID: 2736

UniProt: P10070

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis