ERCC3 antibody (AA 242-261)

Catalog No. ABIN351537

This anti-ERCC3 antibody is a Mouse Monoclonal antibody detecting ERCC3 in WB, ELISA, IHC, IP and IHC (p). Suitable for Human.

Quick Overview for ERCC3 antibody (AA 242-261) (ABIN351537)

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERCC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3G4

Product Details anti-ERCC3 Antibody

Binding Specificity: AA 242-261

Brand: IHC-plus™

Specificity: Recognizes an epitope located between aa242-261 of ERCC-3 (largest subunit of TFIIH).

Purification: Protein G purified

Immunogen: Synthetic peptide corresponding to aa242-261 of human ERCC-3.

Type of Immunogen: Synthetic peptide

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 - 10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunoprecipitation: Immunoprecipitates transcriptionally active TFIIH in a reconstituted assay.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-HCl,  pH 7.5. Sourced from ascites.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ERCC3

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Alternative Name: ERCC3 / XPB

Background: Name/Gene ID: ERCC3

Synonyms: ERCC3, BTF2, GTF2H, RAD25, TFIIH p89, TFIIH, XPB, BTF2 p89, XPBC, TFIIH 89 kDa subunit

Gene ID: 2071

UniProt: P19447

Pathways: DNA Damage Repair