IL7R antibody (pTyr449)

Catalog No. ABIN351553

This Rabbit Polyclonal antibody specifically detects IL7R in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IL7R antibody (pTyr449) (ABIN351553)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL7R antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IL7R Antibody

Binding Specificity: pTyr449

Specificity: A BLAST analysis was used to suggest cross-reactivity with IL-7R from human, mouse and rat sources based on 100 % homology with the immunizing sequence. Cross-reactivity with IL-7R from other sources has not been determined.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:100000), IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:10000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Expect a band approximately 55 kD in size corresponding to phosphorylated IL-7 in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: IL7R / CD127

Background: Name/Gene ID: IL7R
Family: Interleukin

Synonyms: IL7R, CD127, CD127 antigen, IL-7RA, IL-7 receptor subunit alpha, IL7RA, ILRA, Interleukin 7 receptor, CDW127, IL-7R subunit alpha, IL-7R-alpha

Gene ID: 3575

UniProt: P16871

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size