Prolactin Receptor antibody

Catalog No. ABIN351557

The Rabbit Polyclonal anti-Prolactin Receptor antibody is suitable to detect Prolactin Receptor in samples from Human. It has been validated for IHC, WB, IP and IHC (p).

Quick Overview for Prolactin Receptor antibody (ABIN351557)

Target: Prolactin Receptor (PRLR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prolactin Receptor antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Prolactin Receptor Antibody

Specificity: Recognizes the unique sequence of the human prolactin receptor (PRLr) intermediate isoform. Reactivity is confirmed with T47D breast carcinoma cell line.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human PRLR / Prolactin receptor.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation: 10 μg/IP reaction.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Prolactin Receptor

Target: Prolactin Receptor (PRLR)

Alternative Name: PRLR / Prolactin Receptor

Background: Name/Gene ID: PRLR

Synonyms: PRLR, HPRL receptor, PRL-R, HPRLrI, Prolactin receptor

Gene ID: 5618

UniProt: P16471

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus