PARP1 antibody

Catalog No. ABIN355558

This Mouse Monoclonal antibody specifically detects PARP1 in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for PARP1 antibody (ABIN355558)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-PARP1 Antibody

Specificity: This monoclonal antibody specifically detects a 89kD cleavage fragment containing the catalytic and auto modification domain. Species cross-reactivity: Human.

Purification: Immunoaffinity purified

Immunogen: This antibody was developed by immunizing mice with a synthetic peptide containing amino acids near 214/215-cleavage site of human PARP.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: Flo, WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow Cytometry and Western Blot. Western Blot: 1-2 μg/mL. Flow Cytometry: 1-3 μg/test. Positive control: Camptothecin treated HL-60 (12 hr).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1 / PARP

Background: Name/Gene ID: PARP1

Synonyms: PARP1, ADPRT, ADP-ribosyltransferase NAD(+), Adp-ribosyltransferase, ADPRT1, ARTD1, ADPRT 1, Poly(ADP-ribose) synthetase, Poly(ADP-ribosyl)transferase, PARP, Poly [ADP-ribose] polymerase 1, PADPRT-1, PARP-1, Poly (ADP-ribose) polymerase 1, Poly(ADP-ribose) polymerase, Poly[ADP-ribose] synthase 1, PPOL

Gene ID: 142

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location