GST antibody

Catalog No. ABIN356349

This anti-GST antibody is a Mouse Monoclonal antibody detecting GST in WB and EIA. Suitable for Schistosoma japonicum.

Quick Overview for GST antibody (ABIN356349)

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Mouse

Clonality: Monoclonal

Conjugate: This GST antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 9AT106

Product Details anti-GST Antibody

Characteristics: Synonyms: Glutathione S-transferase Tag, GST26-Tag

Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: Purified recombinant GST fusion protein was used to produced this monoclonal antibody.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/3,000. Western blot: 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: Glutathione-S-Transferase (GST)

Background: Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner.Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.Synonyms: GST26-Tag, Glutathione S-transferase Tag

UniProt: P08515